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Giardiasis in Dogs

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Giardiasis is a common parasitic infection in dogs, caused by the intestinal parasite Giardia. Puppies, young dogs, and those with weakened immune systems are particularly susceptible. While some dogs show no symptoms, others may experience malabsorption, foul-smelling feces, chronic diarrhea, and weight loss. The infection can be difficult to eliminate due to the risk of reinfection, requiring antiparasitic treatment and thorough environmental cleaning. Adult dogs may carry the parasite without symptoms, shedding cysts and unknowingly spreading the infection.



Transmission

Infected dogs shed cysts in their feces, which can immediately infect other animals. The cycle from ingestion to shedding in feces takes 5 to 12 days in dogs. Giardiasis is common in crowded environments like kennels or shelters due to high levels of fecal contamination. While cysts found in healthy adult dogs may be insignificant, they are more concerning in vulnerable dogs. Controlling environmental contamination is challenging because the cysts are robust and infectious immediately upon shedding in feces-contaminated soil, food, and water.







Clinical signs

Dogs with giardiasis can show a range of symptoms, from no signs at all to severe illness. Some dogs remain asymptomatic with normal appetite and energy levels, while others develop acute, intermittent, or chronic diarrhea. The parasite damages the intestinal lining, leading to malabsorptive diarrhea, often with mucus, a foul odor, and sometimes a greenish tinge or blood. Other symptoms may include abdominal discomfort, lethargy, decreased appetite, weight loss, and vomiting. While most dogs don’t have a fever, they may be less active. The disease can persist for weeks, with younger or immunocompromised dogs more likely to show symptoms. Many dogs remain asymptomatic carriers without developing illness.







Diagnosis

Diagnosing giardiasis in dogs can be challenging because Giardia cysts are not consistently present in the stool. Standard fecal flotation tests may not always detect these cysts, so a special zinc sulfate solution is often used. Additionally, parasites can be identified through a direct smear of the feces. If giardiasis is suspected, stool samples can be tested for Giardia-specific antigens using methods like direct smears, centrifugal fecal flotation, or antigen detection tests, including rapid tests. PCR detection of Giardia in feces is also becoming more common. It may be necessary to conduct multiple tests over several days to confirm the infection.







Prognosis

With proper treatment, most dogs recover fully from giardiasis. However, debilitated, geriatric dogs or those with compromised immune systems are at a higher risk for complications, including potentially fatal outcomes. It's important to re-test all pets diagnosed with Giardia two to four weeks after completing treatment. Dogs can become reinfected if their environment is not adequately managed. Additionally, refractory infections may occur if the Giardia is resistant to the medication or if the 

treatment was not properly dosed.

Prevention

Preventing and controlling giardiasis is challenging due to the persistence of cysts in the environment and on a dog's coat. To reduce the risk of reinfection or transmission, bathe dogs to remove cysts, dispose of feces daily, and clean surfaces with boiling water or chlorine bleach at a 1:32. Since Giardia cysts are susceptible to drying, keep the environment as dry as possible. Thoroughly clean and dry the pet's living areas before reintroducing them

Note

Bioguard’s Qmini PCR can detect Giardia DNA in 90 minutes at your clinics using feces as samples.

To learn more about Qmini PCR, click here: https://www.bioguardlabs.com/q....

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Avian leukosis virus



Avian leukosis virus (ALV) is a retrovirus that can cause cancer in birds. It is associated with tumor development, decreased fertility, and stunted growth in birds. Additionally, it weakens the immune system of birds, making them more vulnerable to other infections and reducing the effectiveness of vaccinations against other diseases. The virus is primarily transmitted from infected hens to their offspring through embryos, but it can also spread through direct or indirect contact with infected chickens or contaminated objects. Currently, there are no effective vaccines or treatments for this disease, so the most practical approach to controlling it is to identify and remove infected birds from the population.



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Clinical symptoms

Infection with ALV can lead to tumor formation, especially lymphoid leukosis, primarily in the liver, spleen, and bursa. Tumors may also occasionally develop in the kidneys and reproductive organs. The characteristics and frequency of these tumors are influenced by factors such as the virus strain, chicken breed, age, and transmission route.

Common clinical signs include:

  • loss of appetite
  • weakness
  • diarrhea
  • dehydration
  • weight loss
  • depression
  • enlarged bursa and sometimes an enlarged liver

Infected birds may also lay fewer eggs, and while they may not necessarily develop tumors, these symptoms may still be present.



Host immune response against ALV

The host's immune response to ALV begins with the innate immune system, the first line of defense against pathogens. However, ALV has been shown to suppress the host's innate immune response.

- Monocytes are precursor cells for macrophages and dendritic cells (DCs), both of which are essential for innate and adaptive immunity.

- Macrophages play a critical role in innate immunity by clearing pathogens, regulating immune responses, and maintaining tissue integrity through the secretion of pro-inflammatory cytokines when activated by viruses, microbes, or cytokines.

- DCs, as sentinel cells, are key in pathogen recognition, antigen presentation, and T-cell stimulation. In ALV-J-infected bone marrow-derived dendritic cells (BM-DCs), differentiation and maturation are significantly inhibited, resulting in apoptosis due to the abnormal expression of microRNAs.



Diagnosis

Standard criteria include evaluating the patient's history, clinical signs, gross pathological findings, and conducting histological examinations.

In more advanced cases, additional methods such as immunohistochemical testing, standard and quantitative PCR assays, virus isolation, and serological testing are employed.




To learn more about Qmini PCR, click here
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For any direct inquiries, please contact us at: [email protected]

About Bioguard Corporation

The Bioguard is a company focusing on animal disease diagnostic services and products.
Our animal health diagnostic center is the first and only ISO/ IEC 17025 accredited animal disease testing laboratory in Taiwan and China.



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